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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | DOHH2 |
| Accession number | ICLC HTL99022 |
| Brief description | Species: human; Tumor: lymphoma, follicular, B cell |
| Description | Species: human male 60 years old; Tumor: lymphoma, follicular, B cell |
| Depositor | Dr G. Canti, Pharmacology Dept, Milan, Italy |
| Reference paper | Leukemia 1991;5:221-224 - PMID: 1849602 |
| Morphology and growth | continuous culture, grown in suspension, morphology single round cells and clumps |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 3-7 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2 |
| Viruses | Epstein-Barr |
| Properties | CD3- CD10+ CD13- CD19+ CD20+ CD34- CD37+ HLA- EBV+ |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Karyology | (14;18)(q32;q21) chromosomal translocation |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | J Steroid Biochem Mol Biol 1996;57:239-249 - DOI: 10.1016/0960-0760(95)00271-5
- PMID: 8645634
Leukemia 1994;8:1385-1391 - PMID: 8057678 Leukemia 1996;10:1198-1208 - PMID: 8684002 |
| Comments | The viability may be low after thawing |