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Cell line nameDOHH2
Accession numberICLC HTL99022
Brief descriptionSpecies: human; Tumor: lymphoma, follicular, B cell
DescriptionSpecies: human male 60 years old; Tumor: lymphoma, follicular, B cell
DepositorDr G. Canti, Pharmacology Dept, Milan, Italy
Reference paperLeukemia 1991;5:221-224 - PMID: 1849602
Morphology and growthcontinuous culture, grown in suspension, morphology single round cells and clumps
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 3-7 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2
VirusesEpstein-Barr
PropertiesCD3- CD10+ CD13- CD19+ CD20+ CD34- CD37+ HLA- EBV+
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Karyology(14;18)(q32;q21) chromosomal translocation
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Steroid Biochem Mol Biol 1996;57:239-249 - PMID: 8645634
Leukemia 1994;8:1385-1391 - PMID: 8057678
Leukemia 1996;10:1198-1208 - PMID: 8684002
CommentsThe viability may be low after thawing

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