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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | KARPAS-422 |
| Accession number | ICLC HTL99023 |
| Brief description | Species: human; Tumor: lymphoma, follicular, B cell |
| Description | Species: human female 73 years old; Tumor: lymphoma, follicular, B cell |
| Depositor | Dr G. Canti, Pharmacology Dept, Milan, Italy |
| Reference paper | Blood 1990;75:709-714 - PMID: 2297573 |
| Morphology and growth | continuous culture, grown in suspension, morphology round to polygonal cells, growing in single or in small clusters |
| Culture conditions | RPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 5-10 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2 |
| Viruses | Epstein-Barr |
| Properties | IgH/bcl2 fusion gene; CD3- CD10+ CD13(+) CD19+ CD20+ CD34+ CD37+ EBV+ |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Karyology | (14;18)(q32,q21) chromosomal translocation |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | the cells are difficult to culture; viability may be low after thawing |