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Cell line nameKARPAS-422
Accession numberICLC HTL99023
Brief descriptionSpecies: human; Tumor: lymphoma, follicular, B cell
DescriptionSpecies: human female 73 years old; Tumor: lymphoma, follicular, B cell
DepositorDr G. Canti, Pharmacology Dept, Milan, Italy
Reference paperBlood 1990;75:709-714 - PMID: 2297573
Morphology and growthcontinuous culture, grown in suspension, morphology round to polygonal cells, growing in single or in small clusters
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 5-10 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2
VirusesEpstein-Barr
PropertiesIgH/bcl2 fusion gene; CD3- CD10+ CD13(+) CD19+ CD20+ CD34+ CD37+ EBV+
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Karyology(14;18)(q32,q21) chromosomal translocation
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Commentsthe cells are difficult to culture; viability may be low after thawing

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