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Cell line nameMDA-MB-468
Accession numberICLC HTL99024
Brief descriptionSpecies: human, Black; Tissue: breast; Tumor: adenocarcinoma
DescriptionSpecies: human, Black female 51 years old; Tissue: breast; Tumor: adenocarcinoma; Derived from: pleural effusion
DepositorDr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa, Italy
Reference paperIn Vitro 1978;14:911-915 - PMID: 730202
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2; do not allow to reach confluence
PropertiesEstrogen receptor alpha negative. HLA: Aw23, Aw30; B27, Bw35; Cw2, Cw4
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1980;40:3118-3129 - PMID: 7000337

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