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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MDA-MB-468 |
| Accession number | ICLC HTL99024 |
| Brief description | Species: human, Black; Tissue: breast; Tumor: adenocarcinoma |
| Description | Species: human, Black female 51 years old; Tissue: breast; Tumor: adenocarcinoma; Derived from: pleural effusion |
| Depositor | Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa, Italy |
| Reference paper | In Vitro 1978;14:911-915 - DOI: 10.1007/BF02616120 - PMID: 730202 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2; do not allow to reach confluence |
| Properties | Estrogen receptor alpha negative. HLA: Aw23, Aw30; B27, Bw35; Cw2, Cw4 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1980;40:3118-3129 - PMID: 7000337
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