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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MOLT-4 |
| Accession number | ICLC HTL99025 |
| Brief description | Species: human; Tissue: peripheral blood; Tumor: leukemia, T cell |
| Description | Species: human male 19 years old; Tissue: peripheral blood; Tumor: leukemia, T cell; Derived from: a patient with acute lymphoblastic leukemia (ALL) in relapse |
| Depositor | Dr G. Canti, Pharmacology Dept, Milan, Italy |
| Reference paper | J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231 |
| Morphology and growth | continuous culture, grown in suspension, morphology round cells, single or in clumps, lymphoblast-like |
| Culture conditions | RPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 5-10 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2. |
| Properties | G->A mutation at codon 248 of the p53; CD1+ CD2+ CD3+ CD4+ CD5+ CD6+ CD7+ CD19- EBV-; high level of terminal deoxynucleotidyl transferase |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | J Natl Cancer Inst 1980;65;715:718 - DOI: 10.1093/jnci/65.4.715
- PMID: 6932523
Proc Natl Acad Sci USA 1990;87:7555-7559 - DOI: 10.1113/jphysiol.1966.sp007992 - PMID: 16992228 |