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Cell line nameMOLT-4
Accession numberICLC HTL99025
Brief descriptionSpecies: human; Tissue: peripheral blood; Tumor: leukemia, T cell
DescriptionSpecies: human male 19 years old; Tissue: peripheral blood; Tumor: leukemia, T cell; Derived from: a patient with acute lymphoblastic leukemia (ALL) in relapse
DepositorDr G. Canti, Pharmacology Dept, Milan, Italy
Reference paperJ Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
Morphology and growthcontinuous culture, grown in suspension, morphology round cells, single or in clumps, lymphoblast-like
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 5-10 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2.
PropertiesG->A mutation at codon 248 of the p53; CD1+ CD2+ CD3+ CD4+ CD5+ CD6+ CD7+ CD19- EBV-; high level of terminal deoxynucleotidyl transferase
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Natl Cancer Inst 1980;65;715:718 - DOI: 10.1093/jnci/65.4.715 - PMID: 6932523
Proc Natl Acad Sci USA 1990;87:7555-7559 - DOI: 10.1113/jphysiol.1966.sp007992 - PMID: 16992228

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