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Cell line nameLoVo
Accession numberICLC HTL99028
Brief descriptionSpecies: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma
DescriptionSpecies: human, Caucasian male 56 years old; Tissue: colon; Tumor: adenocarcinoma
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperCancer Res 1976;36:467-475 - PMID: 1260746
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsHam's F12 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:2-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
PropertiesCEA production. HLA: A11; B15, B17; Cw1, Cw3.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1979;39:2630-2636 - PMID: 445465
Exp Cell Res 1976;101:414-416 - PMID: 964319
J Natl Cancer Inst 1978;61:75-83 - PMID: 276641

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