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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | LoVo |
| Accession number | ICLC HTL99028 |
| Brief description | Species: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma |
| Description | Species: human, Caucasian male 56 years old; Tissue: colon; Tumor: adenocarcinoma |
| Depositor | Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy |
| Reference paper | Cancer Res 1976;36:467-475 - PMID: 1260746 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | Ham's F12 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:2-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | CEA production. HLA: A11; B15, B17; Cw1, Cw3. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1979;39:2630-2636 - PMID: 445465
Exp Cell Res 1976;101:414-416 - DOI: 10.1016/0014-4827(76)90393-1 - PMID: 964319 J Natl Cancer Inst 1978;61:75-83 - DOI: 10.1093/jnci/61.1.75 - PMID: 276641 |