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Cell line nameY79
Accession numberICLC HTL99029
Brief descriptionSpecies: human, Caucasian; Tumor: retinoblastoma
DescriptionSpecies: human, Caucasian female 2 years old; Tumor: retinoblastoma
DepositorDr F. Tosetti, Molecular Biology Dept, IST at ABC, Genoa, Italy
Reference paperJ Natl Cancer Inst 1974;53:347-360 - DOI: 10.1093/jnci/53.2.347 - PMID: 4135597
Morphology and growthcontinuous culture, grown in suspension, morphology multicellular clusters
Culture conditionsRPMI 1640 + 2mM L-Glutamine + 15% FBS; maintain cultures between 5-9 x 10^5 cells/ml; split 1:2-1:4 (not disaggregate the clusters); 37C, 5% CO2. After thawing the viability of the cells may be low (thaw with medium supplemented with 20% FBS).
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsIn order to obtain a cell monolayer the surface of the flask must be coated with poly-D-lysine 2.5 ug/cm2

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