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Cell line nameAF-2 clone 9B5
Accession numberICLC HYL95001
Brief descriptionSpecies: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: myeloma
DescriptionSpecies: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: myeloma
DepositorDr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa, Italy.
Reference paperUnknown
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 3 and 9 x 105 cells/ml; split 1:5-1:10; resuscitate with medium containing 20% FCS, with a feeder layer of 3T3. 37C, 5% CO2
PropertiesHybridoma producing IgM monoclonal antibody specific for AF-2 protein.
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with G6PD, NP, MD
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Further bibliographyExp Cell Res 1991;193:411-419 - DOI: 10.1016/0014-4827(91)90114-a - PMID: 2004654
J Cell Physiol 1991;149:567-574 - DOI: 10.1002/jcp.1041490328 - PMID: 1744180

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