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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | AF-2 clone 9B5 |
| Accession number | ICLC HYL95001 |
| Brief description | Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: myeloma |
| Description | Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: myeloma |
| Depositor | Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa, Italy. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 3 and 9 x 105 cells/ml; split 1:5-1:10; resuscitate with medium containing 20% FCS, with a feeder layer of 3T3. 37C, 5% CO2 |
| Properties | Hybridoma producing IgM monoclonal antibody specific for AF-2 protein. |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with G6PD, NP, MD |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Exp Cell Res 1991;193:411-419 - DOI: 10.1016/0014-4827(91)90114-a
- PMID: 2004654
J Cell Physiol 1991;149:567-574 - DOI: 10.1002/jcp.1041490328 - PMID: 1744180 |