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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | 7TD1 |
| Accession number | ICLC HYL96001 |
| Brief description | Species: mouse, C57BL/6; Tumor: hybridoma, B cell |
| Description | Species: mouse, C57BL/6; Tumor: hybridoma, B cell |
| Depositor | Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy |
| Reference paper | Proc Natl Acad Sci USA 1986;83:9679-9683 - DOI: 10.1073/pnas.83.24.9679 - PMID: 2948184 |
| Morphology and growth | continuous culture, grown in suspension, morphology single or clustered, round cells |
| Culture conditions | Iscove's modified Dulbecco's medium + 10% FBS + 2mM L-Glutamine + 0.24mM L-asparagine + 0.55mM L-arginine + 0.1mM Hypoxantine + 50uM 2-mercaptoethanol + 25ng/ml IL-6; maintain cultures between 3 and 9 x 105 cells/ml; split 1:5-1:10. 37C, 5% CO2 |
| Properties | IL-6 dependent hybridoma obtained by fusion of spleen cells from a mouse immunized with 50 ug Escherichia coli lipopolysaccharide, indicator cell line for IL-6. |
| Distribution | Cell line available for distribution,only as frozen ampoule, upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Comments | Initially after thawing the viability of the cells may be low |