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Cell line nameMab 62B1-PC
Accession numberICLC HYL98001
Brief descriptionSpecies: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma
DescriptionSpecies: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma
DepositorDr G. Grassi, Experimental Institute for Industrial Cultures, Bologna, Italy
Reference paperClin Exp Immunol 1987;70:192-200 - PMID: 3319299
Morphology and growthcontinuous culture, grown semiadherent, morphology round cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 3-9 x 10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2
Propertiesanti plasmacell hybridoma, obtained by fusion of spleen cells from a mouse immunized with human plasmacells with PX63Ag8-653, a mouse myeloma line; HAT resistant
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as mouse with MD, MPI
Freezing medium90% FBS + 10% DMSO
CommentsThe viability of the cells can be very low after thawing; thaw with medium supplemented with 20% FBS

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