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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | Mab 62B1-PC |
| Accession number | ICLC HYL98001 |
| Brief description | Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma |
| Description | Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma |
| Depositor | Dr G. Grassi, Experimental Institute for Industrial Cultures, Bologna, Italy |
| Reference paper | Clin Exp Immunol 1987;70:192-200 - PMID: 3319299 |
| Morphology and growth | continuous culture, grown semiadherent, morphology round cells |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 3-9 x 10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2 |
| Properties | anti plasmacell hybridoma, obtained by fusion of spleen cells from a mouse immunized with human plasmacells with PX63Ag8-653, a mouse myeloma line; HAT resistant |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with MD, MPI |
| Freezing medium | 90% FBS + 10% DMSO |
| Comments | The viability of the cells can be very low after thawing; thaw with medium supplemented with 20% FBS |