 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | B9 |
| Accession number | ICLC HYL98004 |
| Brief description | Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma |
| Description | Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma; Derived from: B13.29 (first described as clone 9.9) |
| Depositor | Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy |
| Reference paper | Eur J Immunol 1987;17:1411-1416 - DOI: 10.1002/eji.1830171004 - PMID: 3500054 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 50uM 2-mercaptoethanol + 50pg/ml human IL-6 ; maintain cultures between 3-9 x 10^5 cells/ml; split 1:3-1:4; 37C, 5% CO2 |
| Properties | fusion partner: SP2/0-Ag14; non producer hybridoma; IL-6 dependent |
| Distribution | Cell line available for distribution, only as frozen ampoule. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with AST, NP |
| Freezing medium | 90% FBS + 10% DMSO |
| Comments | the cells grow very slowly, particularly after thawing |