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Cell line nameB9
Accession numberICLC HYL98004
Brief descriptionSpecies: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma
DescriptionSpecies: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma; Derived from: B13.29 (first described as clone 9.9)
DepositorDr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Reference paperEur J Immunol 1987;17:1411-1416 - DOI: 10.1002/eji.1830171004 - PMID: 3500054
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 50uM 2-mercaptoethanol + 50pg/ml human IL-6 ; maintain cultures between 3-9 x 10^5 cells/ml; split 1:3-1:4; 37C, 5% CO2
Propertiesfusion partner: SP2/0-Ag14; non producer hybridoma; IL-6 dependent
DistributionCell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as mouse with AST, NP
Freezing medium90% FBS + 10% DMSO
Commentsthe cells grow very slowly, particularly after thawing

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