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Cell line nameHUVEC
Accession numberICLC IST05001
Brief descriptionSpecies: human; Tissue: endothelium
DescriptionSpecies: human; Tissue: endothelium; Derived from: umbilical cord vein
DepositorDr A. Albini, Exp. Oncology A Dept, IST, Genoa, Italy
Reference paperMicrovasc Res 2004;68(2):110-8 - PMID: 15313120
Morphology and growthprimary culture, grown as monolayer, morphology endothelial
Culture conditionsMedium 199 + 10% FBS + 2mM L-Glutamine + heparin 100 mg/L + aFGF 10ug/L + bFGF 10ug/L + EGF 10ug/L + hydrocortisone 1mg/L; split the cells 1:2-1:3 using cold trypsin/EDTA (no more than one minute), seed at 6x10^3 cells/cm2, 37 C, 5% CO2
Properties% of CD31 positive cells: >90%
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsThe cells reach at least passage 12; better results are guaranteed at low passages. Each ampoule contains about 5x10^5 cells. After thawing the viability of the cells may be low. The medium should be kept at 4C, it should never be heated neither left too long at room temperature. The flasks must be presoaked with gelatin 1% in PBS for 30 minutes. After gelatin coating, rinse once with cold PBS. Do not reuse flask. Donors are tested for HBV, HCV, HIV. HUVEC are available from single donors (male or female), and the sex is specified.

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